Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M Tris-HCl Solution, pH 7.4. 500 ml, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 100mM Tris-HCl, pH 8.2. 1000mL, Sterile 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Quality Biological Inc Tris-HCl (1M), pH 7.5, Pack 4 X 100 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride provide convenient and highly purified solutions. Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).
Use Tris-HCl (1M), pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered DNase, RNase and Protease Tested
pH Range: 7.5 ± 0.1
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Research Products International Corp TRIS-Tricine-SDS Running Buffer 10X Solution, 500 ML
10X Formulation:
Tricine: 1 M
SDS (pH 8.3): 1%
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.5) - 2x1L
Bioprocess Grade - Tris-HCl Buffer (1 M pH 8 5) - 2x1L (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, MFCD00132476, 10pk
pH 8.0, powder, Synonym: TBS. Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); pH 8.0, at 25 °C.
Research Products International Corp NP-40 Lysis High Salt Buffer Solution, 500 ML
1X buffer used for extracting proteins from cells.
Nusep Inc NUVIEW UNIV GEL 10 10-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 10% 10well. Cassette size: 10 x 8 cm. Fits Hoefer, Cytiva, Merck Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Zymo Research Corporation ZymoPURE™ Binding Buffer (150 ml)
ZymoPURE™ Binding Buffer (150 ml). Assists in binding nucleic acid to ZymoPURE™ columns for purification.
Biotang Inc Phosphate Buffered RIPA with Glycerol (2X), 125 ml
Phosphate Buffered RIPA with Glycerol (2X), 125 ml.
Kd Medical Inc TBE 10X 1 LITER BOTTLE
Tris Borate EDTA is used for polyacrylamide and agarose gel electrophoresis. 10X TBE is concentrated and ready to be diluted to a 1X working solution. One 1 liter bottle.
Alkali Scientific QuickSilver™ Buffer Powder, Tris-Glycine-SDS Buffer Powder, 50 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
Alkali Scientific CellPro™ Tris HCL, 1M pH 6.8, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.